Electron Energy-Loss Spectroscopy (EELS) is a valuable tool used by researchers to explore the electronic and structural properties of materials at the nanoscale. EELS has been widely used to provide insights on the thickness of materials, obtain chemical composition, and reveal oxidation state and bonding properties of elements. Recent advancements in instrumentation and knowledge of electron-sample interactions have expanded the scope of what can be obtained with EELS, including magnetic and optical properties, molecular vibrations, phonon dispersions, and isotopic detection. This workshop will delve into the foundational principles of EELS and introduce how it can be applied to a broad range of material properties. New data analysis techniques will also be covered. Participants can expect to gain a deeper understanding of EELS and its applications in various fields of materials science. Overall, this workshop presents an excellent opportunity for students and researchers to broaden their knowledge of this powerful technique.

[Topics of Interest]
• Fundamental concepts of EELS,
• EELS applied to the “daily” life of material scientists
• Monochromated EELS applied to optical properties of materials
• EELS used in cryogenic, pyrogenic, and liquid environments.

Atom probe tomography (APT) delivers accurate three-dimensional chemical analysis at a high level of sensitivity for all elements irrespective of their mass and compositions. For instance, imaging hydrogen and lithium inside structural/energy materials with atom probe microscopy have already been studied widely, and this is likely to become even more powerful.

In recent years, with the development of an environmental transferring system and adapted cryogenic preparation protocols, the field of frozen solid-liquid-gas has been opened unravelling structural and chemical evolution of solid-liquid-gas interface. The workshop will bring together leading scientists in atom probe microscopy and related technologies to address important challenges in cryogenic APT experiment particularly sample preparations for high-resolution imaging. This is a forum where experts share their approaches and views and engage in in-depth discussions to resolve challenges and issues.


[Topics of Interest]
• cryo-perservation, instrument advances, and sample preparation/stability
• reconstruction and data analysis of soft matters (frozen liquid, organics, gas-charged materials)
• chemical and cryogenic fixation approaches and cryogenic focused ion beam milling

Many novel materials processes that are critical to the development of renewable energy systems, quantum materials and advanced healthcare solutions, occur on atomic length and time scales. These are exactly the observation scales that are accessible by modern electron microscopes and with the recent development of commercially available in-situ holders, operando experiments have become possible in many locations around the world. However, despite the growing ease of experimental access, there are many easily overlooked requirements that make the generation of reliable, reproducible and quantitative data very challenging. In this workshop we bring together leaders in in-situ microscopy to delve into the precise controls needed to understand how to set up a microscope and perform quantitative experimental observations using 3 of the most common types of in-situ stages – biasing, gases and liquids. The workshop will cover all practical considerations and common mistakes for each type of experiment and provide participants with a guideline to performing their own state-of-the-art experiments.


[Topics of Interest]
• Dose Control and Signal/Noise Issues for In-situ EM
• The Fundamental Control |Parameters for In-situ heating/Biasing
• Measuring and Controlling Gas Reactions in the SEM/TEM/STEM
• From Assembling Liquid cells to Quantitative Electrochemistry Experiments

Artificial Intelligence (AI) and machine learning (ML) methods have become widely used across virtually all sectors of society. In this tutorial, we will focus on an introduction to AI/ML Methods as applicable to microscopy data, from electron and scanning probe microscopy generally. The day will start with an introduction to common methods and challenges associated with application of AI/ML algorithms to microscopy. Then we will cover image and spectral methods for better understanding spectroscopic datasets, e.g. from STEM-EELS or from hysteresis curves, etc. Finally, the workshop will focus on the use of deep learning methods for structure-property relationships, including the use of variational autoencoders and methods to add invariances for more physically-interpretable latent space representations. Finally, the workshop will conclude with a vision for use of AI/ML towards autonomous microscopy systems as physics discovery platforms.


[Topics of Interest]
• machine learning
• spectral processing
• image processing
• physics extraction
• autoencoders

Many experiments in scanning / transmission electron microscopy (S/TEM) collect an enormous amount of data, due to the widespread adoption of high speed direct electron detectors. These include in situ time series, tilt series for 3D tomography, spectroscopy data such as STEM-EELS, and four dimensional STEM (4D-STEM) diffraction experiments. As a result, we require fast and efficient software codes to analyze these large datasets. This workshop will provide an overview of open source python tools for analysis of atomic resolution imaging, spectroscopic, and diffraction datasets. In this workshop, we will direct participants through hands-on python tutorials and provide example datasets for analysis of 4D-STEM experiments using py4DSTEM. We will also explain the principles behind simulation of TEM and STEM experiments, and lead participants through tutorials of the abTEM and Prismatic simulation programs.


[Topics of Interest]
• Overview of analysis of imaging, diffraction, and spectroscopy TEM experiments with python.
• py4DSTEM analysis of 4D-STEM, including strain, orientation, DPC, and ptychography.
• Simulation of diffraction patterns with py4DSTEM.
• Simulation of atomic resolution STEM experiments with Prismatic.
• Simulation of atomic resolution TEM experiments with abTEM.

Many functional materials and devices inherently possess 3D structures, which often contain interfaces, surfaces, voids and defects at different lengthscales ranging from microns to atomic levels. Since the materials and device properties can strongly be influenced by these complex 3D structures, electron tomography has been widely used as a tool for exploring the morphologies, compositions and physical properties of materials in 3D. Recently, with the aid of rapidly evolving electron optics and detector technology as well as advanced reconstruction algorithms, the electron tomography technique has achieved enhanced spatiotemporal resolution with multi-dimensional modalities from 4D-STEM capabilities and spectroscopic tools. This symposium invites contributions that address the forefront development of tomography techniques in hardware and software aspects, together with recent achievements based on these techniques.


[Topics of Interest]
• Electron tomography in atomic resolution
• Tomography combined with spectroscopy: EDS and EELS tomography
• Tomography based on ptychography
• Development of novel hardware and algorithms for electron tomography
• 3D structure and related materials property characterizations based on electron tomography

The central quantities of interest in materials and life science microscopy leave their fingerprint in the phase of the electron wave. Since the phase gets lost during the recording, the development of phase retrieval and phase contrast techniques is key to map electric fields, magnetic induction and the structure of weakly scattering objects. In the last decade, 4D-STEM imaging as the natural evolution of differential phase contrast (DPC) imaging has proven highly versatile to retrieve electrical and magnetic properties, to achieve superresolution and to invert multiple scattering in specimens. This workshop first introduces to the experimental details of 4D STEM incl. data recording, interpretation and processing. In particular, the design of virtual detectors for angular multi-range analysis, centre-of-mass imaging and DPC is explained by means of practical examples. Second, the workshop addresses the inverse problem and introduces to several concepts of phase retrieval using ptychographic concepts. In that respect, reconstruction schemes are presented for both single-scattering cases and for inverting dynamical diffraction.


[Topics of Interest]
• 4D-STEM data acquisition, processing and interpretation
• Measurement of electric and magnetic fields by 4D-STEM and DPC
• Fundamentals of the inverse problem in electron diffraction
• Ptychography approaches for single scattering
• Inverting dynamical scattering by ptychography

This pre-congress workshop will focus on advances in time-resolved electron microscopy and new findings using the method. Studies on photonics, physical, chemical, and materials dynamics spanning attoseconds to microseconds and instrumentation for accessing those timescales are highly welcome. In addition to communicating instrumentation developments and the new scientific advances resulting from ultrafast (and fast, single-shot, and fast-frame) electron microscopy (transmission and scanning) experiments, a goal of this workshop is to stimulate discussions on future directions of time-resolved electrn microscopy and to foster the formation of new collaborations and new ideas within the relevant communities.


[Topics of Interest]
• Techniques and instrumentation for high-resolution (spatial and temporal) imaging, in stroboscopic, single-shot, an fast-frame modes.
• Scientific findings on structural, electronic, and magnetic dynamics enabled by using time-resolved electrn microscopy methods.
• Photonics under strong photon-electron interactions that has been enabled by ultra-brief optical and/or electron pulses, coherent control of quantum system, electron-state manipulation.

Our understanding of the 3D structure and functional subtleties of complex biological systems has skyrocketed due to recent advances in EM imaging technology and hybrid methodologies. This symposium will highlight structural studies of macromolecules, microorganisms, cells, and tissues using state-of-the-art high-resolution techniques. These techniques include single particle cryo-EM, cryo-electron tomography, helical reconstruction, and molecular modeling. Biological topics of interest include cellular architecture, structure and function of memrane proteins, and structure and function of dynamic molecular machines.


[Topics of Interest]
• single particle cryo-EM
• cryo-Electron tomography
• Correlated Light and Electron Microscopy (CLEM)
• molecular modeling

Super-resolution microscopy, a field awarded the Nobel Prize, has transformed the world of optical microscopy by overcoming the diffraction limit of light and enabling noninvasive observation of the nanoscale world. It includes stochastic optical reconstruction microscopy (STORM), photoactivated localization microscopy (PALM), structured illumination microscopy (SIM), and stimulated emission depletion (STED) microscopy. These techniques have been further developed through interdisciplinary advancements in optics, chemistry, and software, demonstrating improved imaging performance in spatial resolution and throughput. Super-resolution microscopy is now essential for discovering the nanoscale morphology of biological specimens, enabling new biological studies and applications. This workshop will bring together leaders in the super-resolution microscopy field to share and discuss its current status and future directions, providing valuable insights for both microscopy developers and users.


[Topics of Interest]
• Development of super-resolution microscopy techniques, including fluorescent probes and labeling strategies, optics and imaging systems, and image analysis and data processing
• Multimodal super-resolution microscopy techniques, including correlative imaging and spectroscopic imaging
• Super-resolution imaging of (live) cells, tissues, and other non-biological specimens
• Application of super-resolution microscopy, including protein interactions and dynamics, nanoscale morphology, disease mechanisms and drug discovery

Expansion microscopy (ExM) improves the resolution of fluorescence microscopy by physically expanding specimens using swellable hydrogels. This technique involves embedding specimens in a hydrogel and homogenizing them, followed by expansion in DI water. Since its first demonstration in 2015, ExM has been widely adopted to solve problems in various biological applications, including neuroscience, cancer, developmental biology, and even diagnostics. The workshop covers the basic molecular mechanism of ExM, examples of its use, differences between different hydrogel and anchoring chemistries, state-of-the-art expansion techniques, and practical considerations for implementing ExM.


[Topics of Interest]
• Various expansion microscopy chemistries and strategies
• Practical guidelines for the implementation of expansion microscopy
• State-of-the-art expansion microscopy techniques and their applications
• Application of expansion microscopy to diverse model systems, including C. Elegans, zebrafish, and mouse embryos

This workshop aims to explore the basic principles of EBSD and TKD analysis techniques. Providing information about how the choice of analysis parameters effects the data, exploring considerations which should take place before data is being acquired in order to get good data in a time efficient manner. This is combined with application examples showing how EBSD & TKD can be effectively used in the different developing application areas.


[Topics of Interest]
• Introduction to EBSD and TKD
• The effect of SEM conditions and analysis parameters, how choices can affect the data.
• EBSD applications in Battery industry, Electronics industry and Materials Science

The workshop aims to introduce the basic theory of Raman spectroscopy and its applications in various fields. To aid in the attendees' understanding, we plan to share case studies from invited speakers and conduct a live Raman demonstration at the end of the workshop.


[Topics of Interest]
• 3D Raman Imaging & Correlative Microscopy techniques
• RISE Microscopy – Combining Chemical Imaging with Ultrastructural and Elemental Analysis
• Advances in Raman Imaging Tools and Spectral Data Evaluation – Showcase and Demo

SPM New Technology and Option Introduction & Hands-on Experience

SPM: Scanning Probe Microscopy
- PinPoint Mode
- Sideband KPFM
- DFRT PFM
- Lithography

Atomic Force Microscopes (AFM) are capable of imaging samples with exceptionally high resolution. Atomic defects and the DNA double helix can both be resolved using advanced AFM systems. In this workshop, we will introduce attendees to AFM fundamentals, ultra-high resolution imaging, and advanced AFM modes. We will demonstrate ultra-high resolution AFM performance and ease of use on the Cypher ES Atomic Force Microscope.


[Topics of Interest]
• Atomic Force Microscopy (AFM) Fundamentals
• Ultra-High Resolution Imaging with AFM
• Advanced AFM Modes of Operation

The workshop aims to introduce participants to a new generation of scientific microscopes called holotomography with insightful presentations covering the underlying technology, its technical development, and the advantages that make it a powerful tool to tackle current scientific challenges. Audiences will also be engaged in deeper discussions on a wide range of applications and be inspired to approach their research topics in practical ways.

The majority of microscopy techniques in live cell imaging limit the quantity and quality of information available to researchers, constrain the study of thicker specimens, and even harm the cells during long-term studies. Tomocube provides a ground-breaking solution to overcome these limitations, allowing work with confluent cell sheets, significantly reducing laser-induced speckle noise for enhanced contrast, delivering enhanced levels of fluorescence illumination, and enabling the study of thicker specimens. Tomocube’s high-resolution, high-sensitivity, high-throughput solution was designed to meet the growing demand for live cell imaging tools in the emerging fields of cell therapy, drug development, cell organelle analysis, etc.