Battery sample preparation: Observe air-sensitive materials in their native state with the CleanConnect and CleanMill Systems
Most materials used in clean energy applications are sensitive to air and water, especially battery materials. The Thermo Scientific IGST (Inert Gas Sample Transfer) Workflow protects your samples to allow you to focus on your research rather than look for sample contamination due to improper sample handling.
Presenter: Brandon van Leer

Room 217
Room 218

Unlocking the secrets of the cell: A high-throughput collaborative in situ cryo-ET project
Cryo-electron tomography (cryo-ET) has become the preferred method for investigating the structures of biomolecules in their native context. However, the current method of producing thin sections through cryo-focused ion beam milling has limitations that can compromise data quality and volume. A next-generation cryo-plasma FIB improves both lamellae quality and throughput, combining with recent advances in data collection and processing to help you collect large in situ datasets in a reasonable time frame.
Presenter: Xianjun Zhang

Room 217

Unfold the nanoscale: Explore cutting edge innovations in TEM imaging & system automation.
As the scientific horizon evolves, so do the challenges we face. In the rapidly evolving landscape of electron microscopy, the Talos system emerges as a groundbreaking integration of cutting-edge technologies, setting new benchmarks for imaging capabilities. This presentation aims to provide a comprehensive overview of the unique features and capabilities that Talos brings to the table. Central to our discussion will be the integration of 4DSTEM, a powerful technique that allows for in-depth diffraction studies, enabling researchers to gain detailed insights into nanoscale structures. Complementing this is the X-CFEG component, designed to produce an exceptionally bright and stable electron beam, ensuring that imaging is consistently of high resolution and clarity. Beyond these technical features, a notable strength of Talos lies in its user-centric design. We will delve into the system's advanced automation capabilities, which not only simplify complex tasks but also enhance the efficiency and accuracy of operations. By combining state-of-the-art technology with user-friendly features, Talos stands as a testament to the future potential of electron microscopy. Join us as we unpack these innovations, shedding light on how Talos is shaping the trajectory of scientific research in microscopy.
Presenter: Cigdem Ozsoy-Keskinbora

Room 218

Vacuum transfer system for EM application combined with a cryogenic technique
Low-carbon efforts to realize a sustainable society are being vigorously pursued around the world.
Secondary batteries, which are recognized as one of the most important next-generation energy technologies, are urgently needed not only for mobile applications such as smartphones and laptops, but also to further enhance their functionality for use in electric vehicles and other transportation technologies in order to promote low-carbon society.
Among these, lithium-ion battery materials, which are attracting attention because of their high energy density, react with water in the atmosphere when exposed to air, resulting in deterioration. In most cases, however, the sample is exposed to the atmosphere when it is transported to the electron microscope after preparation, making the observation itself difficult. Even if the sample can be transported to the electron microscope without being exposed to the atmosphere, it must be observed under cooled conditions because the crystal structure of the material tends to change due to electron irradiation during observation.
In order to conduct research on such samples, it is essential to develop a multifunctional system that can perform the entire transport process from sample preparation to electron microscopic observation under non-atmospheric conditions, as well as observation under cooled conditions. Against this background, Mel-Build has been working on the development of non-exposure and sample cooling techniques to meet the technical requirements of electron microscopy applications. In this presentation, we will introduce our new TEM holder with non-exposure cooling and Peltier Cooling stage for FIB-SEM, which have been developed based on our accumulated technologies.

Room 317

micro PREP PRO : New Vistas for Failure Analysis and Sample Preparation
The microPREP™ PRO enables laser-based sample preparation for various specimen preparation applications. It complements existing approaches to sample preparation, such as ion beam processing. A choice of laser sources is available for installation to enhance the individual preparation process. microPREP™ PRO is suited to ablate metals, semiconductors, ceramics, polymers, and compound materials. You will receive a short introduction to laser-based sample preparation in this workshop. You will see how easily and quickly this sophisticated system can prepare samples with minimum space requirements.

Room 315

Empowering Innovation in Science with TESCAN Solutions
Find out our newest solutions and talk to our experts. TESCAN presents TENSOR - The first near-UHV 4D-STEM that is Integrated, Precession-Assisted, and Analytical, solutions for Battery research, the newest solutions for Life sciences like Pushing Boundaries in Structural Analysis and TESCAN's Correlative Laser Ablation and Xe Plasma FIB-SEM Workflow for semicon applications.

Room 315

Introduction of the latest FE-SEM techniques for Efficient Automated Workflow and Ultralow-Voltage Observation
The latest Hitachi FE-SEMs, SU8600/SU8700, incorporates multifaceted imaging, automation, enhanced system stability and efficient workflows for users of all experience levels. An ultra-low voltage application for battery materials and an automation data acquisition capability will be presented.
Presenter: Shigeaki Tachibana

Room 215

In-situ S/TEM analyses supporting new energy-related material developments
This presentation will introduce recent examples of solutions for observing catalysts in oxidation and reduction processes and for analyzing a lithium-ion battery with the ”Lab.-in-gap”, environmental TEM: HF5000, in which practical gaseous atmosphere and temperature can be simulated to elucidate reaction mechanisms and deterioration factors.
Presenter: Hiroaki Matsumoto

Room 215

3D Correlative Live and Cryogenic imaging of Biological Tissues: Structural Analysis of the Zebrafish Scale
Imaging biological tissues at different length scales (e.g. correlative light and electron microscopy (CLEM)) has shown its strength in the last decades, by combining dynamics and high-resolution imaging with fluorescent-LM and EM, respectively. For EM, ultrastructure preservation is essential and is traditionally done with chemical fixation and resin embedding. However, these chemical agents can induce artefacts. Instead, physical fixation is shown to be a powerful method allowing for high resolution imaging of samples in the hydrated native stage. Cryo-CLEM is already a well-established method on 2D cell culture, but is unexplored on for volume imaging on 3D samples such as tissues and organoids, which are more biological relevant.
Here, I will present a new 3D cryoCLEM workflow for tissue imaging connecting live-cell fluorescence with volume cryo-FIB/SEM. To connect the two modalities, cryo-fluorescence was used to localize the live-cell imaged region of interest in the high-pressure frozen carriers/samples. For high accurate localization of LM to FIB/SEM, we developed a tailor-made finderTOP, which leaves a grid pattern on the ice surface that can be imaged with both modalities. This approach allows high resolution live-cell imaging to be combined with 3D cryo-FIB/SEM volume imaging and eventually cryo-lamellae lift-outs can be made for cryoTEM.In addition we demonstrate the integration of Raman microscopy in our workflow to provide (bio)chemical information correlated with the 3D structural information of the tissue.
We currently explore this workflow to study matrix development in bone organoids-on-chip, focusing on the organization and mineralization of collagen.
Presenter: Nico Sommerdijk

Room 314

Introducing the latest advancements in our SEM solutions portfolio: Live chemical imaging and automated particle analysis
Presenter: Chris Stephens

Room 217

Amira Software: Get actionable results from your biological imaging data
Thermo Scientific Amira Software is a powerful, comprehensive, and versatile 2D–5D solution for visualizing, analyzing, and understanding life sciences and biomedical research data from many image modalities. With incredible speed and flexibility, Amira Software supports advanced 2D–5D bioimaging workflows in research areas ranging from structural and cellular biology to tissue imaging, neuroscience, preclinical imaging, and bioengineering. From any 3D image data, including time series and multi-channel, Amira Software delivers a comprehensive range of data visualization, processing, and analysis capabilities. Amira Software allows life sciences and biomedical researchers to gain invaluable insights into their data, at different scales and from any modality.
Presenter: Jiyoung Park

Room 218

4D STEM in practice with DECTRIS ARINA
As 4D STEM becomes a staple TEM characterization approach, practical use questions become as relevant as the ones related to detector technology and performance.
Aiming at both novice users and experienced microscopists, this after-hour session will focus on the practical use aspects of DECTRIS ARINA detector in the context of fast 4D STEM experiments.
It will encompass topics ranging from the optimum TEM setup and relevant data acquisition parameters to the output data and metadata handling, storage and results extraction.
Join us for a practical exchange on 4D STEM, we look forward to share and learn from your experience.
Coffee & Food Catering will be provided

Room 316

In-situ S/TEM analyses supporting new energy-related material developments
This presentation will introduce recent examples of solutions for observing catalysts in oxidation and reduction processes and for analyzing a lithium-ion battery with the ”Lab.-in-gap”, environmental TEM: HF5000, in which practical gaseous atmosphere and temperature can be simulated to elucidate reaction mechanisms and deterioration factors.
Presenter: Hiroaki Matsumoto

Room 215

Introduction of the latest FE-SEM techniques for Efficient Automated Workflow and Ultralow-Voltage Observation
The latest Hitachi FE-SEMs, SU8600/SU8700, incorporates multifaceted imaging, automation, enhanced system stability and efficient workflows for users of all experience levels. An ultra-low voltage application for battery materials and an automation data acquisition capability will be presented.
Presenter: Shigeaki Tachibana

Room 215

Latest innovations in DualBeam FIB-SEM technology for materials science
For over 30 years, Thermo Fisher has been pioneering DualBeam (FIB-SEM) technology. Our instruments enable researchers and engineers to accelerate science and innovation thanks to improvements in sample preparation and large volume 3D analysis which are often used for advanced materials characterization. Join this session to learn more about the most recent DualBeam developments in the field of automated workflows, Plasma FIB technology and integrated fs-Laser.
Presenter: Mikhail Dutka

Room 217

X-ray microscopy in plant biology--Multiscale 3D imaging above and below the soil line
Imaging in plant biology is a critical aspect of understanding growth and development, particularly for meristems and inflorescence structures. In addition, understanding the interaction of roots with soil is crucial, but it is difficult as these processes occur underground. Capturing detailed 3D image data of these delicate structures and complicated environments is difficult for conventional imaging. X-ray microscopy (XRM) bridges this imaging gap by providing multiscale high-resolution 3D tomographic data where cell-level volumes can be situated within the context of the whole structure. XRM is a powerful tool for visualizing root-soil-microbe interactions in situ without having to dig up the root system. Correlative imaging is also significantly enhanced by using XRM of whole resin-embedded plant samples as a road map for nanometer-scale volume electron microscopy, avoiding the uncertainty of sectioning blindly through a sample. Use of XRM in the multiscale and multimodal analysis of 3D plant features using economically and scientifically important plant systems will be presented.
Presenter: Keith Duncan

Room 314

Latest updates for Smart EPU and Tomography Software: Increasing automation and ease of use for cryo-EM data acquisition
Preparation of thin, Ga-free S/TEM samples using focused ion beam (FIB) milling is still considered a challenging task that requires a high level of expertise. The Thermo Scientific Helios 5 Hydra FIB-SEM, in combination with Thermo Scientific AutoTEM 5 Software, significantly simplifies the workflow and enables automated processing of high-quality, Ga-free lamellae for a broad range of materials. Join us to see how the Helios 5 Hydra FIB-SEM's unique capability to tailor the ion species to specific materials for best results, along with its low-energy polishing (down to 500 eV), makes it best in class for site-specific TEM sample prep.
Presenter: Fanis Grollios

Room 217

Avizo Software: Get actionable results from your materials imaging data
Analyzing and visualizing imaging data can help you better understand your material's structure, properties, and performances. No matter what scale and data modality you use, Thermo Scientific Avizo Software provides optimized workflows for advanced materials characterization and quality control from a single environment. Avizo Software is a suite of universal, reliable, fully automatable, and customizable digital analytical labs that help materials scientists and engineers innovate faster, produce more reliable processes, and develop better performing materials while reducing cost and time to discovery.
Presenter: Jiyoung Park

Room 218

Glacios 2 Cryo-TEM and Smart EPU Software: Streamlining single-particle data acquisition
In this presentation, the new Glacios 2 cryoTEM system will be discussed, its new software and hardware features as well as latest applications results. In addition, a unique pharmaceutical case study will be presented in which the Glacios 2 features as a critical instrument in the SPA workflow for faster drug development.
Presenter: Marc Storms

Room 217

Embark on your characterization journey with Maps Software
Scientists and researchers rely increasingly on nanoscale observations to inform the latest advances in research and analysis. It has, however, become apparent that high-resolution observations lose much of their utility in the absence of the larger macroscopic context. Observations from multiple sources must be linked, providing the necessary multi-scale and multi-modal insight for truly valuable data. Thermo Scientific Maps Software provides powerful imaging workflow automation within an easy-to-use and robust platform. With just a few clicks, you can collect impactful data while preserving the context of your observations. Join this short talk and learn how Maps Software provides an integrated approach to automated data acquisition, annotation, and storage across multiple microscope platforms and modalities.
Presenter: Greg Clarke

Room 217